I am using lectin-based ELISAs to probe the glycans of different glycoproteins.
I had successful data that showed clear differences in the lectin binding profiles/patterns.
However, I wanted to ask if I needed to do a S/N ratio for all the lectins with the different glycoproteins? Is this really a necessary optimization step when designing lectin ELISAs for glycoproteins?
I would use checkerboard ELISAs as they were the most common and straightforward method. Then from the data, choose which range of glycoprotein concentrations were giving the best S/N from the best concentration of lectin as well
i.e., glycoprotein 1 had a good S/N concentration if it was in the 100-200ug/ml range and with lectin 1-8 at 5-10 ug/ml range. Basically, choosing the best S/N taking to account all lectins.
Thanks in advance for your advice!
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Plating