How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Have you ever observed membrane damage during ultrafiltration (microcon-30 centrifugal filters - Ultracel PL30) by centrifugation?

We performed glycopeptide enrichment by lectins and deglycosylation of peptides on MICROCON centrifugal filters (30 kDa, Ultracell PL 30). Checking for the peptide yield by Tricine PAGE, we...

04 April 2019 4,531 8 View

Replacement for erythrocytes (RBCs) for carrying out lectin - hemagglutination assays?

For an experiment I have to test for presence of lectins in a plant extract. I shall be carrying out the regular hemagglutination assay. However, I wish to avoid the use of erythrocytes. So I want...

03 April 2019 2,854 1 View

Have you ever observed membrane damage during ultrafiltration (microcon-30 centrifugal filters - Ultracel PL30) by centrifugation?

We performed glycopeptide enrichment by lectins and deglycosylation of peptides on MICROCON centrifugal filters (30 kDa, Ultracell PL 30). Checking for the peptide yield by Tricine PAGE, we...

03 April 2019 8,529 9 View

Sources for open access Impatiens images (see below)?

Posting on behalf of Wojciech Adamowski :-) Sources of open access Impatiens images? I am looking for sources of color images of correctly determined Impatiens species. I am looking...

31 December 2018 681 2 View

In order to design gene specific primer for Lactose/Galactose binding lectin in a coleopteran (order) insect, can I rely on database sequences (aa)?

I am working on cloning the lactose/galactose (galectin) binding lectin in my insect model of coleopteran system. Apart from the mass spectrometry based results, if I can retrieve the database...

30 October 2018 4,965 5 View

In order to design gene specific primer for Lactose/Galactose binding lectin in a coleopteran (order) insect, can I rely on database sequences (aa)?

I am working on cloning the lactose/galactose (galectin) binding lectin in my insect model of coleopteran system. Apart from the mass spectrometry based results, if I can retrieve the database...

09 October 2018 7,138 6 View

What is the role of lectins in the soil?

What is the role of lectins in the soil? In our studies we found in the soil proteins that have agglutinating activity. Their evaluation showed that they are lectins. Like soil enzymes, they may...

08 September 2018 5,257 4 View

Hello , researchers , i need to find a protocol to evaluated the toxicity of mushrooms lectins in mice ou rats to complet my expierement ?

does any one worked on lectins toxicity in mice or rats

06 July 2018 6,652 0 View

T-cell receptors interacting specifically with acetylpeptides?

Hi, Is anyone aware of any publications, in which T-cells with T-cell receptors interacting specifically with acetylpeptides but not unmodified peptides, were reported? Thank you. Best, Martyna

05 June 2017 1,657 1 View

Looking method for sperm protein fractionantion?

02 March 2017 5,519 2 View