LCA study or technical information about xylitol production through chemical route?

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What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Help to isolate Ralstonia from plant tissue?

I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...

02 July 2024 9,807 3 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

I'm having trouble extracting total RNA from a culture of macrophages derived from monocytes infected with Leishmania infantum. Can someone help me?

I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...

12 May 2024 1,045 4 View

How can I set the distance between peptide and bilayer using Packmol?

I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...

29 April 2024 8,231 1 View

What requirements (or qualities) are most important to be a good university professor?

22 April 2024 3,009 5 View

How to calculate the solute concentration in molecular dynamics simulation?

I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...

16 April 2024 2,051 2 View

What are the factors contributing to the phenomenon of plant roots exhibiting a purple coloration?

In my doctoral research, i'm investigating the intersection of biotechnology and maize seed (Zea mays) productivity. An intriguing observation I have made pertains to the coloration of maize seed...

09 April 2024 1,726 6 View

How has the efficiency of photovoltaic cells improved over time?

On the use of solar energy in homes.

07 April 2024 9,465 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

The use of data from PubChem for commercial purposes?

Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...

30 July 2024 8,707 1 View

I'm conducting a study on Research Commercialization in Pakistani HEIs and would appreciate your participation in a brief survey?

Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...

18 July 2024 7,024 1 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

Use magnetic bead purification after gel electrophoresis?

Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...

25 June 2024 971 2 View

Which LCA software would you recommend for comparative LCA of electrolysers? And is ecoinvent database alone is enough for such a assessment?

For additional information, I am a master's student looking for a LCA software to do my thesis. So a less expensive one is my first priority, but if it is not able to help me with a clarity...

18 June 2024 8,376 3 View

I am trying to do LCA of Water Treatment Technologies. Are there any recommended introductory resources or textbooks on LCA that you suggest?

Some Other Questions, if you can answer: What types of data are essential for conducting an LCA, and where can I find reliable data sources for water treatment technologies? How do you deal with...

11 June 2024 4,791 0 View

Maritime Transport also know as search Ocean transport?

Maritime transport, also known as sea or ocean transport, is the process of moving goods and passengers over water using ships and other vessels. It is a crucial component of international trade,...

08 June 2024 8,981 2 View

How to explain the difference in LCIA results from ReCIPe2016 and IMPACT World+ with literature?

Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...

03 June 2024 8,431 2 View

Is there a study on the LCA of Kapton/Polyimide available?

As Kapton is one of the predominantly used substrates in printed electronics applications I was wondering if there is a study on the LCA of Kapton/Polyimide available? At least considering the raw...

21 May 2024 7,402 0 View