I prepared my seeds extracts in hexane and DCM, now I facing problems in LCMS/MS, it blocks the system, there is any best or ideal mobile phase for both non-polar extracts so that nonpolar constituents can be easily identified by LCMS/MS
You have not included any information about the HPLC method you have chosen. The "Method" should be appropriate for the sample(s) and without detailed column, mobile phase, sample, injection and running conditions, no constructive suggestions can be made.
- What does the statement; " it blocks the system " mean? Are you referring to a clog or obstruction? If so, precipitation may be occurring due to using solvents which do not fully dissolve the sample or material.
If your concern has to do with the solution that your sample(s) are dissolved in (i.e. n-Hexane or DCM), then dilute the sample in the mobile phase. Tiny amounts of injection solution (relative to the column volume) should not have any quality effects on the chromatography. For HPLC, the injection solution should elute at To, far away from your sample.
Properly developed HPLC methods should be used for all samples. Their is no universal mobile phase, column or system (their are millions). Perhaps the best way for you to address your concerns would be to speak to a professional chromatographer at your school who is experienced in developing HPLC methods for the type of MS system that your school has (using both NP and RP modes).
I think that hexane and DCM are not preferred solvents for LC/MSMS samples, because they may not mix properly with your aqueous mobile phase. Methanol would be better. In addition, you should dilute your extract e.g. 1:100 with mobile phase and centrifuge it before injection.
Try to partitionate your extract. Prepare using hex and dcm, after that dry the extract using rotatory evaporator and then use methanol to redissolve your extract.
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