Muqeet Wahid

4 Questions 2 Answers 0 Followers

Questions related from Muqeet Wahid

LC-MS/MS for n- hexane and DCM extract of seeds?

I prepared my seeds extracts in hexane and DCM, now I facing problems in LCMS/MS, it blocks the system, there is any best or ideal mobile phase for both non-polar extracts so that nonpolar...

21 December 2020 2,521 3 View

Primer Design for real time PCR?

I am working on primer designing and had the following questions 1. which primer design software is the best primer quest or NCBI primer blast 2. When I design a primer on primer quest, I see Tm...

29 August 2020 7,668 4 View

How to kill the rats for organ bath in vitro studies?

Hi, Normally Rats killed by cervical dislocation to get the smooth muscle or tissues. But cervical dislocation is very painful for rats. there is anesthetia i give them prior to kill which may...

29 July 2019 3,418 5 View

What will be the phytochemical in successive extraction of Seed ?

I am using 4 solvents in following order (n-hexane, DCM, Chloroform, Methanol, ) to get maximum phytochemicals for pharmacological activities. Another question is in above which extraction is...

20 January 2019 9,422 4 View