Hi all, my lab image analyzer LAS-3000 I use for western blotting is not cooling down to -30 degrees; it is getting stuck at -23. Is there any trick or way to solve it?
I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...
11 February 2021 1,136 3 View
Hello I have modelled RC beam in ANSYS apdl. first create volume, then create keypoints & create a line joining that keypoints to make reinforcement. I am working on thermal analysis, for...
20 January 2021 7,344 2 View
Hello I want to add thermal convection load on specific area of concrete volume. it asks for film coefficient which is a constant value but the bulk temperature has no. of values that i have as...
18 January 2021 6,627 1 View
The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.
27 December 2020 7,325 2 View
i am working on thermo-mechanical analysis of RC beam using ANSYS. i want to input temperature dependant stress strain curve in ansys. i tried using elastic > multilinear but a warning attached...
23 December 2020 9,928 2 View
Actually I am developing IoT based pollution monitoring system and need some ideas which help in developing great product with great features
10 October 2020 5,657 7 View
As per the latest research, Microsoft finds underwater data centers are reliable, practical and use energy sustainably so how can we enable IoT with LoRa technology in the underwater data center...
05 October 2020 535 4 View
I have taken 1000 PPM of nanoparticles in 1 ml of RO water and pursuing its UV visible spectro absorbance scan. They are showing respective peaks on the baseis of energy transition of electrons...
19 September 2020 6,276 3 View
I work at a precious metal refinery. We extract gold using aqua regia. However, sometimes Gold does not dissolve in AR. Sometimes it precipitates due to cold weather? Is there a way to make sure...
07 August 2020 7,059 3 View
Hello everyone, I want to know whole chemistry behind synthesis of boron nanoparticle via chemical and biogenic methods. Focussing on why naked boron is not used as I have seen in papers, boron...
05 August 2020 1,195 14 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...
03 March 2021 5,857 3 View
i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).
03 March 2021 7,100 1 View
Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru
02 March 2021 1,787 2 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...
01 March 2021 9,874 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...
27 February 2021 8,128 1 View
Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...
27 February 2021 1,194 3 View