I would like to check the physical interaction between a protein and drug in vitro. Can anyone suggest the best method to study protein-drug interaction and where in India I can outsource this facility(including protein purification).
Hello Charles,
I’m research scientist at Bioxtal a biotech company provider for protein expression and purification and binding assays. Since more than fifteen years, we developed expertise in structural biology and drug discovery projects. We have projects with academic and industrial research labs around the world. You can contact me for a no-obligation, competitive quote on your project. In order to check physical interaction between protein and drug we use mainly isothermal titration calorimetry (ITC), microscale thermophoresis (MST), differential scanninf fluorimetry (DSF), biolayer interferometry (BLI) and we can develop different enzymatic test to test drug inhibition.
U can start with the evaluating the inhibition of enzyme activity to see the effect of the drug. Once you confirm inhibition, u should check the binding of drug to the enzyme using ITC, CD, fluorescence anisotropy/polarization etc. u can also perform docking studies of the drug on the structure/model of the protein followed by molecular dynamic simulation in order to understand the structural flexibility and dynamics of the protein upon drug binding. check some recent papers from our group, they may be helpful for your work.
https://www.ncbi.nlm.nih.gov/pubmed/28627969
https://www.ncbi.nlm.nih.gov/pubmed/28605994
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