Charles K Davis

11 Questions 8 Answers 0 Followers

Questions related from Charles K Davis

Can anyone help me identify the proteins from the mass spectra?

In order to see the binding of a drug to proteins of interest, I used DARTS assay. In the 1D gel, one lane was loaded with crude protein sample and the other lane was loaded with crude protein...

16 February 2018 7,368 3 View

Labs in India where protein-drug interaction studies are possible?

I would like to check the physical interaction between a protein and drug in vitro. Can anyone suggest the best method to study protein-drug interaction and where in India I can outsource this...

28 June 2017 4,383 2 View

Which is the most cost effective way to study Protein-drug interaction in vitro?

I need to analyze the interaction/binding of a drug to a protein. Protein overexpression and purification seem to be every expensive. Is there any way I can study protein-drug binding/interaction...

04 April 2017 3,325 3 View

Anyone would like to share SK-N-SH or SH-SY5Y cell line in India?

My lab would like to procure SK-N-SH or SH-SY5Y cell line. Thanks in advance.

01 November 2016 3,436 2 View

Gromacs: Residue not found in residue topology database ; How to fix this?

My protein source file (pdb) contains FE2+ (co-factor) and 2-oxoglutarate (co-substrate) but none of the force fields can incorporate them in while giving for MD simulation.

05 April 2016 2,667 4 View

Can we target substrate binding site of an enzyme for docking studies?

Is it a good strategy to generate the grid at the substrate binding site of an enzyme? Later I'll be using this grid to screen a small molecule database to identify the potential inhibitors. My...

26 January 2016 7,078 3 View

What value is considered as a promising docking score in Schrodinger software?

How I'll know whether the protein and the ligand are binding strongly from the docking score? Is there anything like a minimum/maximum docking score which I should get to confirm the strong...

15 August 2015 210 12 View

What should be the SH-SY5Y cell density for doing MTT assay after Oxygen Glucose deprivation?

I am planning to do MTT assay after Oxygen Glucose deprivation (OGD) and drug treatment for SH-SY5Y cell line. What will be the appropriate cell density per well (96 well plate) for seeding?....

06 December 2014 5,727 5 View

Is it compulsory to exclude all Cq/Ct values more than 30?

Does error (difference of less than 3 degree Celsius) in annealing temperature have any role in getting a higher Cq/Ct?

15 September 2014 5,029 12 View

Does someone know how to plot a "Timber plot" (not sure about the name), it is different from "forest plot"?

Meta-analysis: Timber plot. Please see the attachment.

26 August 2014 7,596 2 View

Can anyone help with RNA isolation from Neuroblastoma cells?

Any one using "innuPREP DNA/RNA Mini Kit" from Analytik Jena fro RNA isolation? I have a problem, tried isolating RNA from SH-SY5Y cells but failing at the first step itself. Not getting any...

14 August 2014 4,163 3 View