Charles K Davis

13 Questions 11 Answers 0 Followers

Questions related from Charles K Davis

Can anyone help me identify the proteins from the mass spectra?

In order to see the binding of a drug to proteins of interest, I used DARTS assay. In the 1D gel, one lane was loaded with crude protein sample and the other lane was loaded with crude protein...

16 February 2018 7,390 3 View

Labs in India where protein-drug interaction studies are possible?

I would like to check the physical interaction between a protein and drug in vitro. Can anyone suggest the best method to study protein-drug interaction and where in India I can outsource this...

28 June 2017 4,423 2 View

Which is the most cost effective way to study Protein-drug interaction in vitro?

I need to analyze the interaction/binding of a drug to a protein. Protein overexpression and purification seem to be every expensive. Is there any way I can study protein-drug binding/interaction...

04 April 2017 3,357 3 View

Anyone would like to share SK-N-SH or SH-SY5Y cell line in India?

My lab would like to procure SK-N-SH or SH-SY5Y cell line. Thanks in advance.

01 November 2016 3,453 2 View

Gromacs: Residue not found in residue topology database ; How to fix this?

My protein source file (pdb) contains FE2+ (co-factor) and 2-oxoglutarate (co-substrate) but none of the force fields can incorporate them in while giving for MD simulation.

05 April 2016 2,692 4 View

Can we target substrate binding site of an enzyme for docking studies?

Is it a good strategy to generate the grid at the substrate binding site of an enzyme? Later I'll be using this grid to screen a small molecule database to identify the potential inhibitors. My...

26 January 2016 7,098 3 View

What value is considered as a promising docking score in Schrodinger software?

How I'll know whether the protein and the ligand are binding strongly from the docking score? Is there anything like a minimum/maximum docking score which I should get to confirm the strong...

15 August 2015 228 12 View

Why in the MTT assay the Formazan crystals are not dissolving?

This is what I did: Seeded the cells in 96 well plate (8000cells/well, 150ul). 24hrs incubation Oxygen Glucose Deprivation (OGD) for 5 hrs After one hour addition of drug 18 hrs incubation Added...

04 June 2015 7,811 10 View

What will be the best antibiotic product?

What will be the best antibiotic product (trade name or brand) for neuroblastoma (SH-SY5Y) cell culture? something which can resist mycoplasma.

17 December 2014 1,765 1 View

What should be the SH-SY5Y cell density for doing MTT assay after Oxygen Glucose deprivation?

I am planning to do MTT assay after Oxygen Glucose deprivation (OGD) and drug treatment for SH-SY5Y cell line. What will be the appropriate cell density per well (96 well plate) for seeding?....

06 December 2014 5,757 5 View

Is it compulsory to exclude all Cq/Ct values more than 30?

Does error (difference of less than 3 degree Celsius) in annealing temperature have any role in getting a higher Cq/Ct?

15 September 2014 5,078 12 View

Does someone know how to plot a "Timber plot" (not sure about the name), it is different from "forest plot"?

Meta-analysis: Timber plot. Please see the attachment.

26 August 2014 7,618 2 View

Can anyone help with RNA isolation from Neuroblastoma cells?

Any one using "innuPREP DNA/RNA Mini Kit" from Analytik Jena fro RNA isolation? I have a problem, tried isolating RNA from SH-SY5Y cells but failing at the first step itself. Not getting any...

14 August 2014 4,185 3 View