I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!
Dear Khaled
There are several procedures for protein purification and identification. So search in the literature for one depending on the nature of your protein. The easy technique is ion-exchange chromatography on a column. Following purification, it is convenient, as the first step of characterization, to estimate the protein apparent molecular mass on PAGE, in native conditions, and denaturing conditions using SDS.
Dear Joseph,
Thanks a lot for your answer and help.
What we need exactly is to repeat a study was done by us years ago to separate certain fractions of honey protein using salting out and test these fractions versus GS-9L then using HPLC to get the active protein and to test this protein or proteins again versus GS-9L.
As we did this before, we isolated some of these proteins but we still think of presences of another protein or peptides active versus cancer cells.
So, can you help?
Please, look at patents:
https://www.google.com/patents/WO2014040605A1?cl=en
https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2017182044&recNum=1&maxRec=&office=&prevFilter=&sortOption=&queryString=&tab=PCT+Biblio
We exactly need it as a paid service from a trusted proteins lab.
Unfortunately, we do not have enough time to do that ourself nowadays.
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