Hi folks,
Currently, I made construct to generate version 3 CAR-T cell. Before IVT cell, I tested expression in 293t cell. It was good and highly expression.But not express in T-cell. Does anybody know what happened or any experience in this situation? Thank you all.
Junjie Shao
from google:
T lymphocytes are considered as a hard to transfect primary cells. - T cells are resistant to common chemical transfection reagents, such as Lipofectamine 2000, polyfect and TurboFect. - These chemical transfection reagents are less popular in the case of T cell transfection due to their low transfection efficiency
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