I need to stain the hippocampus of mice brain with KI antibody. I tried different dilution of AB and retrieval with EDTA and citrate. But i still get week positive result. What can i change to improve the result??
Marco Ghibaudi
Dear Duaa,
I assume you mean Ki-67 antibody, right?
In our lab, we perform an antigen retrieval in citrate buffer pH 6 at 90°C. For mice, 5' of this treatment should be enough. If not, try to increase the time up to 20'/30'. The dilution used by us is 1:500.
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