Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Is there a certain way of making HEPES buffer w/ KCl?

I am trying to switch from NaCl to KCl in my buffer. As soon as I add 4M KCl solution to DI water and HEPES, it precipitates. Would heating the buffer alleviate the problem? Or should i add KCl to...

07 January 2021 1,104 1 View

What are some of the differences between a research article and a review article?

What are some of the differences between a research article and a review article? Thanks in advance for your replies!

29 December 2020 2,066 26 View

For experts in Gaussian 09 for windows?

Dear Friends: 64 bit Gaussian 09 software for windows is more expensive than 32-bit Gaussian 09. In our work, we deal with coordination compounds with about 50 atoms. So, can we use 32-bit...

27 December 2020 2,877 2 View

Suggestions for Journals related to Psychology and Computer Science?

I am looking for a journal, related to Psychology and Computer Science. IF < 2.0. If anyone has published some related work, or know any journal. that would be nice.

07 December 2020 1,277 3 View

FEM modeling of Joints/ Connection?

The attached Joint.png showed and example case of a joint (2 continuum members are connected). There are 3 characteristics of this, 1. There is a Gap: Cause small amount of rigid body rotation and...

07 December 2020 6,603 3 View

How to compute intermolecular interactions in mixture of solvent?

Hello Is it possible to compute intermolecular interactions in a mixture of solvent using DFT tools in Gaussian-09 or GAMESS US, DMOl3 or any other computational chemistry code? For example we...

05 December 2020 7,336 3 View

How can we set up Hydrothermal Liquefaction using solar/microgrid for extracting biofules?

Fo the conversion of biomass to biofuels, Hydrothermal Liquefaction is chosen.But the whole operation has to be from renewable energy source including the whole process most probably by solar and...

23 November 2020 9,432 2 View

Do you think that the emergence of CORONAVIRUS affects Investor trading behaviors? How? give examples?

any Thoughts on this question will help me Thanks

06 November 2020 7,654 4 View

Dynamics of cochlea membrane ?

04 November 2020 3,333 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View