Small holes in TEM section?

I am working on cells for TEM, and i follow my regular procedure , but i still gets small holes in the cell and resin around, i tryed to increase the time of infiltration but the section did not...

19 December 2018 5,792 14 View

How can we connect fins to the back surface to the solar cell?

If I have Aluminum fins L shape and copper fins with 5 mm thickness , how I can paste it on the rear surface of the PV panel to cooling it by natural convection ?

09 August 2018 2,070 7 View

How untransfected still survive against G814?

19 September 2017 4,271 3 View

Problem in converting data from Excel to SPSS?

I want to convert the data from excel to SPSS 20.so far I convert it but now facing problem in Data Type.I want to convert from string to numeric for analysis and while doing so all of the data...

10 March 2017 9,730 7 View

What volume should be used of 2X Mastermix ?

I have PCR master mix 2X (Thermoscientific).How much volume of this mix should be used in 25ul reaction volume & should it be diluted to 1X

27 February 2017 9,228 3 View

How can I simulate disulfide bond formation using MD?

Although it is familiar in molecular biology, I could not found it in literature. Using QM/MM is known to stimulate bond formation and breaking, however, how to drag this reaction to take place in...

11 May 2014 6,864 1 View

He architectural and urban heritage of all cities is better than the current output of architects and urban developers

The current product of architects and urban developers will it be at one time a legacy for our future generations and they will be proud of it or will they criticize it and leave it and blame us...

01 January 1970 8,837 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View