the aim is to identify a region in genomic DNA that flanking a known sequence.
What are the realistic applications of self-compacting geopolymer concrete in the world? Are there names of projects implemented with this concrete around the world? Please mention the names and...
20 June 2024 6,040 2 View
in transmission line differential protection this a big issue
28 May 2024 1,676 1 View
In recent research area, what about of advanced techniques?
28 May 2024 324 2 View
Can you help me? I'm doing my first research and I can't find papers about this object.
23 May 2024 1,410 5 View
suggest research regarding this topic
17 May 2024 9,366 0 View
Hi, I have to do GFP immunohistochemistry on a rodent brain tissue at 400um thickness, I have a protocol that works quite well for 40um thick sections which involves overnight incubation at room...
25 March 2024 4,740 1 View
Many times co-authors do not respond due to lack of time, disagreement, personal commitments and personal problems in life. Can one make a decision to exclude them (such co-authors) from the...
18 February 2024 3,819 6 View
( paid ). I need someone speaking Arabic tell me how can do a steel frame with slab concrete subjected to blast load by Abaqus prog.
02 February 2024 644 3 View
Sustainable production of chemicals, pharmaceuticals, and clean water
02 February 2024 1,594 0 View
Hi I am interested in multi-agent systems, and I want to start reading and reserach on multi-agent systems. I need textbooks to lerarn the basics. Also, any papers about recent advenaces...
26 November 2023 5,702 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View