Just to refresh your knowledge: Speciﬁc methods for studying the protein composition of tissues such as?

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Alternative promoters have role in all the following except? a) tissue specificity b) developmental activity c)expression levels d)growth?

Just to refresh your information

10 November 2016 9,738 2 View

What is the Example of Loss of speciﬁc chromosomes in somatic cells?

Just to refresh your mind 1- Example of Loss of speciﬁc chromosomes in somatic cells could found in:- a) Human b) Nematode c) Monkey d)Soy bean

10 November 2016 5,383 2 View

What is the definition of Monomorphic, polymorphic or unique bands in gel image?

How we can define in simple words the differences between Monomorphic, polymorphic or unique bands on gel image can from gel electrophoresis

08 September 2014 612 9 View

Are there any resources to read about in silico methods to discover retrotransposons?

There are many complete sequenced genomes, it needs a lot of efforts to discover the contents and the fine structures of those genomes. I am looking for an effective and easy method to mine the...

06 July 2014 7,560 3 View

Can the analysis of X-Ray Images and MRI Images be considered bioinformatics?

By a narrow sense wide sense of the term, it is open discussion.

11 December 2013 1,193 8 View

Are genomic retrotransposable elements ancient integrated viruses in the genome?

Open discussion

10 November 2013 9,105 11 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View