What is the Example of Loss of speciﬁc chromosomes in somatic cells?

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Alternative promoters have role in all the following except? a) tissue specificity b) developmental activity c)expression levels d)growth?

Just to refresh your information

10 November 2016 9,738 2 View

Just to refresh your knowledge: Speciﬁc methods for studying the protein composition of tissues such as?

1- Speciﬁc methods for studying the protein composition of tissues such as:- a) SDS-PAGE b) Western- blotting c) Northern-blotting d)Southern blotting

10 November 2016 2,856 2 View

What is the definition of Monomorphic, polymorphic or unique bands in gel image?

How we can define in simple words the differences between Monomorphic, polymorphic or unique bands on gel image can from gel electrophoresis

08 September 2014 612 9 View

Are there any resources to read about in silico methods to discover retrotransposons?

There are many complete sequenced genomes, it needs a lot of efforts to discover the contents and the fine structures of those genomes. I am looking for an effective and easy method to mine the...

06 July 2014 7,560 3 View

Can the analysis of X-Ray Images and MRI Images be considered bioinformatics?

By a narrow sense wide sense of the term, it is open discussion.

11 December 2013 1,193 8 View

Are genomic retrotransposable elements ancient integrated viruses in the genome?

Open discussion

10 November 2013 9,105 11 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How many established theories of macroevolution?

There are seven (7) established theories of macroevolution viz. i) Chromosomal speciation theory, ii) Shifting balance theory, iii) Punctuated equilibrium theory, iv) Allopatric speciation theory,...

10 July 2024 9,679 1 View

How to extract good quality RNA from Plant parasitic nematodes?

Hi, I am dealing with plant parasitic nematodes. I tried extracting RNA from almost 1000J2 worms with two different kits (Qiagen RNeasy Kit, Omega RNA extraction Kit) and by two different ways of...

09 July 2024 3,742 2 View

Designing Inclusive City. What's your opinion regarding my group's campaign below?

Hello! I have a campaign assignment related to inclusive cities. Would you mind to take a look and give your opinion regarding my group's campaign? What kind of public facilities do you think are...

07 July 2024 368 0 View

How your consciousness can have an after-life?

For evolution to act on the conscious process it must be expressed as a behavioral output (Dawkins 1976). So, thinking about an idea without writing it down or saying it will not do. So far, an...

07 July 2024 5,867 0 View

Does anyone know any C++ implementation of Kolmogorov-Arnold network other than mine?

Mine is sitting here: http://openkan.org/DLpiecewiseCPP.html I wish to see someone else's.

06 July 2024 9,974 1 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View