I'm trying to quantify by WB some proteins from plasma exosomes but I'm not finding a proper loading control. Does anyone know wich protein can I use?

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How to perform a Qualitative Analysis of Polarization on Twitter ?

Hi, I want to study the increasing polarization on social media. I have to use a qualitative method to do so. However, I am more familiar with quantitative methods. Hence, I am struggling to find...

15 January 2021 2,426 3 View

Using different scales for intra-organisational findings?

Hi all, I am trying to assess 3 unrelated factors in our organization. Together, they should give me an index of how well our workers in different countries are prepared to continuously learn...

22 November 2020 3,495 3 View

Have you tried the Tissue Total RNA Mini Kit, with DNase (50) rxns from Geneaid?

We are going to work with very small tissue samples and we are looking into different kit from many brands and this one is significantly less expensive, but we haven't heard about it. Has anybody?...

18 November 2020 1,387 1 View

Troubles with cryosectioning (frozen sections) on skin tissues?

Can someone help me with some cryosectioning issues? I made skin tissues in vitro and my protocol is already validated. I've always go for the paraffin cutting but is currently out of service....

05 October 2020 4,339 10 View

Comparing correlated means in a t-test?

Hello, I have a categorical variable (main teaching assignment) with 12 options (early childhood, mathematics, English, etc) and I am trying to determine which groups earn significantly higher...

08 September 2020 5,046 5 View

Does anyone know a rat cell line which expresses EpCAM?

I am looking for a model cell line to validate anti-rat EpCam Antibodies but i am struggling to find a good candidate. Many thanks!

24 August 2020 218 2 View

How do I interpret my AGE results?

Hello! I am in need of help analyzing my results from Gel Electrophoresis. The first photo indicates that there was DNA extracted but did not go down. The second one shows that the DNA has been...

07 August 2020 6,125 3 View

Interaction-effect with categorical dependent variable in SPSS?

Hi there, I am struggling with my thesis. I need to test in SPSS if there is a significant interaction effect. I have four groups, and they all fill in a survey. The only difference of the...

27 July 2020 6,074 1 View

How to avoid loss of solvent using Dolomite Microfluidics System and maintain stable flow throughout the run?

Hi everyone. I'm currently working with Dolomite Microfluidic system for preparation on PLGA based microparticles, but I seem to be encountering some issues which have been giving me some trouble...

29 June 2020 827 1 View

After running Chi-square test for comparison between 4 groups, is there a method of checking which groups differ significantly with nominal data?

My data is nominal/categorical. I want to see if 4 groups differ significantly. The question for them is if they convict a person or if they acquit the person. So the data is categorical: convict...

08 June 2020 5,208 7 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View