I'm trying to quantify by WB some proteins from plasma exosomes but I'm not finding a proper loading control. Does anyone know wich protein can I use?

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Triet Bui

CD63 and Annexin A1

Júlia Abbade Tronco

Hi, but aren’t these proteins’ expression variable depending on the cell of origin?

Nothing is perfect. But the guideline markers for exosomes are CD63 and CD81.

Ok, thank you! I have the CD63 antibody so I’ll try to use it. Hopefully it’ll work!! Thank you for the help!

Good luck with your scientific endeavors!