Could you please explain more about your question. For example what kind of explants have your tried and what was the response you got. Based on your information I can give some suggestion if you are interested.
Dear sir,thanks for your Question,I used internode and leaf explants from invitro raised plts,i got the callus from 24d+NAA when i cultured in liquid medium and solid medium contain glutamine and ascorbic acid the explts not responding and brown colored and also not getting regeneration from callus(organogenesis) pls give the needful answer sir