Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Do I have the right PCR cycler settings?

Hello. Someone designed primers and cycler settings for me and it doesn't work. I do hot start PCR with touchdown, I detect Babesia sp. DNA in Acari. The primers are F: GYYTTGTAATTGGAATGATGG, R:...

01 March 2020 3,949 3 View

How do I determine differences between two hemp seed samples to determine if they are genetically different strains or the same strain?

Are there currentlly any methods developed for determining the differences in genome between two samples?

08 August 2019 4,594 2 View

My cells started to form clumps during passaging. I tried almost everything to reduce them. Do you have any suggestions how to solve this problem?

My cell cultures (HeLa S3, mouse kidney cells, WB-neo, WB-ras) strarted to form clumps during passaging so it is not possible to count them properly. I TRIED 1. make PBS without Mg and Ca...

14 January 2019 9,398 6 View

How to remove the Percoll from the sample?

I use density gradient centrifugation with Percoll to extract bacteria from the bentonite sample. It is working quite well, but I need to remove the Percoll from the bacterial extract at the end...

25 July 2018 981 3 View

How influence the concentration of oxygen growth of oxide layer and properties of oxide layer itself due to thermal oxidation?

Hello, I'm working on my diploma work on thermal oxidation of TiNb alloy.In my work I have oxidized titanium by two procedures thermal oxidation in laboratory atmosphere and in atmosphere of...

22 May 2018 1,704 5 View

F.I.S.H probe: Can be used as a probe a pcr labeled product with 600-1000 bps size?

Is it nessessary to be inserted in a BAC clone?

05 June 2016 3,512 1 View

Does anyone know whether a chromatography mode exists when both the stationary and mobile phase (developing solvent in TLC) are nonpolar?

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10 October 2012 8,485 11 View

Stimulation of CD8+ CTL with specific antigen in vitro

How to increase effectivity of CTL stimulation with synthetic peptide epitopes.

13 October 2011 2,226 1 View

Nominal vs actual concentration exposure of drugs to aquatic organisms

Hello, Do you know where I can find any critics on ecotoxicological studies which are focused on exposure aquatic organism to drugs? I am looking for papers that critic actual exposure...

01 January 1970 3,981 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View