N-(2-Hydroxypropyl) methacrylamide (HPMA)

Hi there anybody worked withN-(2-Hydroxypropyl) methacrylamide (HPMA) polymer? I wanna capped my gold nanoparticles (5-30 nm diameter) by HPMA polymer, 3.3 kD. any protocol? from beginning to end....

04 May 2017 7,643 4 View

Could anybody give me an idea about Dot blot problem?

Hi... I expressed a scFv antibody in HB2151 e.coli by TES (Tris-EDTA-sucrose) buffer and now like to confirm it by Dot blot, however, I failed to visualized it using DAB.I coat the scFv (with a C...

02 March 2017 4,782 12 View

2TY media preservation

Hi there How many times could 2TY broth be preserved in the refrigerator (4 c)? I mean 2TY broth without glucose and ampicillin. Can I use it after 1 month or more? what

01 February 2017 5,117 2 View

PCANCTAB 5C phagemic copy number ?

hello every body I am using one of pCANTAB vectors (pCANTAB 5C) for my project. there is not details about this type of vector. how can find its details genetic map? since it originated from a pUC...

01 February 2016 5,089 1 View

What is the negative control for panning protocol?

Hi there, Im doing panning protocol with Nunc immunotube according to nature protocols. i coat one immunotube with 50 micro gram/ml of the peptide and one immunotube with no petide and all the...

08 September 2015 4,768 2 View

Gold nanoparticle aggregation challenges ?

Hi As we know AuNPs have surface plasmon resonance (SPR) property. when we modified or functionalized AuNPs with either short or long polymers, does it impact on its SPR properties? Note that some...

01 January 1970 5,339 2 View

How can i disperse Gold nanopowder in polar or non-polar solvents?

Hi there I wanna conjugate a water miscible polymer to a 15 nm spherical gold nanopowder. since the nanogold is not soluble in water, can I dissolve the nanogold powder into non-polar solvents...

01 January 1970 5,792 7 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

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23 February 2021 4,014 3 View