2TY media preservation

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Kathryn Marjorie Jones

If you are certain of the sterility and the glucose and ampicillin have not been added yet, >1 month is fine.

If you are doing a growth curve, the same batch of medium should be used for all of the strains being tested so that they will be grown under equivalent conditions.

Seyed Nooreddin Faraji

Thanks all for invaluable comments. When we re-autoclave our 2TY broth, the color of the media changed to deep brown. is it OK?