i´m looking for a the manner to add oxycholesterol (25-hidroxycholesterol for example) in feed on shrimp culture. i have already seen how to add in cells media but not in animals feeds.
Fish food should ideally provide the fish with fat (for energy) and amino acids (building blocks of proteins) and the fish food (whether flake or pellet) must be speedily digested in order to prevent buildup of intestinal gas, renal failure and infections (such as swim bladder problems and dropsy) and to avoid aquarium pollution due to excessive ammonia. Aquatic diets for carnivores must contain vegetable matter such as spirulina.
Building block ingredients of fish food[edit]
Amino acids are the basic components of proteins. Protein requirement vary according to species .carnivorous fish need a greater percentage of proteins then herbivorous An example of an aquatic diet that is a good source of amino acid is a crumbled hard boiled egg offered to small fry. Large amounts of DL-Methionine enhance the headgrowth of the Lionhead goldfish.
Fats that are broken down into fatty acids are the main source of energy in fish especially for the heart and skeletal muscles. Fats also assist in vitamin absorption. Vitamins A, D, E and K are fat-soluble or can only be digested, absorbed, and transported in conjunction with fats.
Carbohydrates are molecular substances that include sugars, starches, gums and celluloses. Most of the carbohydrates that are incorporated into aquatic diets are of plant origin and are sources of the enzyme amylase. Carbohydrates, however, are not a superior energy source for fish over protein or fat but digestible carbohydrates do spare protein for tissue building. Unlike in mammals, glycogen is not a significant storage depot of energy in fish.
Assuming that oxycholesterol is adequately fat soluble, you can first mix it with an appropriate oil and than add this oil to the other ingredients of your shrimp feed.
C.A. Kan is right. You can add it directly to your oils mixture and its going to dissolve well, although it may take some time depending on the amount you want to add. Alternatively, you can pre-dissolve it in a small amount of ethanol and then add the ethanol to the oil. The ethanol is going to evaporate when you dry the feed.