Hi,

maybe this publication could help:

https://doi.org/10.1016/j.ab.2003.10.047

Btw, you could also "stain" standard SDS-PAGE gels by soaking in TCE after running the gel, but then there is not much of an advantage left compared to other staining methods. The method also works with Trichlor-acetate (only after the run, because TCA can't be added to the gel directly before the run due to its charge).

I guess the imaging system has been optimized in terms of wavelength, exposure time and image processing so if you can afford it, you can save some time. Or spend some time at a regular imager and optimize yourself.

Hope that helps.

Once i transfer protein to membeane ns and wet with methanol to get a transilumination effect. I could see the total protein around the wet methanol. Then could do western Immunostaining. 

Hi Victoria, I noticed that it has been past 3 years. I now have the same question.

So I want to ask that how did you solve this problem? Can you get the total-protein image by other UV capable imager, not Bio-rad ChemiDoc?

Because our WB imager is not Bio-rad ChemiDoc. But I have stain-free gel, I wondering if I can detect total protein by our Amersham Imager.

Victoria C. Kennedy and Qi Wang did you able to solve the problem of imaging with other gel-docs?

I want to use stain free gel too but we have azure imaging system and not Bio-rad, so it would be nice if you share your experience.

Thanks.

I have never used any commercial products for stain free, and just a normal transilluminator. In my case, it is all about the amount of auto-fluorescence of the membrane. Generally speaking, nitro is ok, regular PVDF is bad

Paolo Tenti Thanks for response, could you tell just by visualizing through transilluminator, how do you proceed with band quantification?

Thanks