I'm trying to do total protein quantification with Stain-free precast gels and after a lot of reading and many phone calls with Bio-rad, I am confident it's possible to read the gels and membranes with just UV box or an imager with UV capabilities. Has anyone done this before? How have you been able to get good images and measurements? So far, using a G-Box imgaer has given me pretty bad pictures, but I can see the bands so much clearer on just a regular old UV transilluminator box in my lab. Any suggestions for getting better images for normalizing total protein? 

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