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Questions related from Manisha Shahu
I want to determine the colocalization of a transmembrane protein along with a membrane marker, could it be possible to obtain the result with fluorescence microscopy alone or it is must to have a...
04 July 2023 2,682 5 View
Hi, I am encountering a problem with protein expression and than detecting on western blot. I have around 120kDa membrane bound protein which is now fuse to GFP(~150kDa) to help in selecting...
14 March 2023 6,117 1 View
Hi, I am trying to analyze the protein expression of 2 different proteins via western blot. Since both are different isoform I am using two different antibodies for each respectively. However, in...
16 January 2023 2,810 4 View
Hi, I am trying to do a PCR from transfected HEK cell containing my protein of interest, however I am not getting any success, while the control DNA is giving the correct band with same primers...
23 November 2021 7,527 4 View
Hi, I am working on a membrane bound protein, but due to experimental approach of checking its activity I cannot solubilize my protein and thus I do not use any detergent in my lysis buffer....
03 May 2021 9,276 2 View
Hi, I am trying to create a stable cell line with my protein of interest inserted in vector expressing GFP. However my protein is not directly attached to GFP expressing region. I would like to...
04 January 2021 1,147 5 View