Hello! So we are purifying some barcode DNA after PCR. I am using the QIAquick PCR/Gel clean-up kit, we confirm our product and begin to dissolve our gel/sample, check pH and then add isopropanol. What is the purpose of adding isopropanol? Also, a buffer that requires that enthanol is added to it, which is later used to wash our column once dna is bound to it, this flow through is discarded. What is the ethanol's purpose?
Thank you in advance for your help!
Best,
Ell