I want to isolate Salmonella from meat, which enrichment media could be used selenite or tetrathionate broth?
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200009
The following procedures will guide you through the steps necessary to isolate Salmonella confidently from faeces and from food, carry out a biochemical identification of Salmonella and serotype the strains using slide agglutination tests
https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070149.htm
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070149.htm
The recovery of salmonellae from egg products was studied, by use of three different enrichment procedures: (i) selenite broth, (ii) selenite broth containing 10% sterile feces, and (iii) the lactose pre-enrichment procedure. Brilliant Green Agar was used throughout as the recovery medium. Although the lactose pre-enrichment methodology promoted Salmonella recovery from samples containing small numbers of dormant organisms, the efficiency of this enrichment method is adversely affected by unfavorable coliform-Salmonella ratios. Under such conditions, early subculture of lactose broth into selenite broth is indicated. Selenite broth containing 10% sterile feces was more efficient than the lactose pre-enrichment methodology in promoting the growth of “dormant” salmonellae. Albumen adversely affected recovery of salmonellae from selenite broth, whereas whole egg and egg yolk enhanced Salmonella recovery from this medium. The selenite-feces medium presents a solution to the major problems encountered in the detection of salmonellae in egg products and offers an approach to a single medium in which food-borne salmonellae will manifest themselves with a minimum of laboratory manipulation.
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