is there alternative additive instead of glycerol with same protecting result for long term storage of bacteria isolates?
Dear Alla,
we used ceramig beads suspended in a special cryogenic preserving solutions (e.g. http://www.copanusa.com/products/lab-supplies/cryobank/).
The advantage is that you can remove only one ceramic bead with your bacterium and the rest of bacterial culture remains frozen.
Good luck. Marcela
DMSO is commonly used instead of glycerol at about 5% final concentration.
Hi: You can also use freeze dryer (Lyophilization ) method to preserving bacteria for long term.
It depends on the microbes you are trying to preserve, some bacteria are much more fragile than others and most of those are probably killed by freezing and some of them don't keep as long with freeze-drying (lyophilization).
It's depends on how long you want to preserve the bacteria and also the type of it. If you want to save it for 2-8 weeks, you can store it in freezer with temperature 6 degrees but for longer preservation, lypholization is the best method
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