I am looking for a functional protocol to isolate PBMC from mice blood. The protocols I have seem to produce a low yield of PBMC or are focused on human blood
what can we say is the the Impact of Renewable Energy Integration on Grid Reliability in Nigeria , with examples and possible solution
15 February 2024 3,082 1 View
I am interested in researching on FEM
10 March 2023 7,032 0 View
Below are some of the troubleshooting technique I tried. 1. Baking the column 2. Changed the liner 3. Cut the column 4. Increased injection temperature 5. Changed the Septum Still this negative...
29 September 2021 8,417 5 View
Hello, I am trying to do methanol calibration in gas phase. What I did was to vaporize liquid methanol in a vail and then take some amount with a syringe and inject . I am having weird data. My...
02 March 2021 8,169 2 View
am working on analyzing the amount of HMF I have in the organic phase I use for my reaction( The organic phase is MIBK and 2-Butanol). I am currently doing low-pressure gradient. Find below more...
16 July 2018 1,143 6 View
RSM learning RSM application RSM optimization
13 March 2018 7,589 5 View
Am working on ways of reducing the toxicity of Amphotericin B without affecting the bioavailability.
18 November 2017 321 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View