you also can take a look to this article, authors illustrate and explain very well the technique

https://www.researchgate.net/profile/Lei_Cai11/publication/265848610_An_Optimized_Protocol_of_Single_Spore_Isolation_for_Fungi/links/551e31580cf29dcabb03a371.pdf

Article An Optimized Protocol of Single Spore Isolation for Fungi

He, Here is some useful paper: 

http://www.tandfonline.com/doi/full/10.3852/15-226

https://cals.arizona.edu/classes/plp427L/lab2.html

http://www.coloss.org/beebook/II/fungal/1/4/1/3

http://www.biotechniques.com/BiotechniquesJournal/2010/January/Isolation-of-fungal-homokaryotic-lines-from-heterokaryotic-transformants-by-sonic-disruption-of-mycelia/biotechniques-184894.html

start with monospore isolation that would be easier I think 

you can use saline spore solution using detergents or salt solution 

there is another way is that you proceed by serial dilution (go to 10 - 4) to tip single conidies then you tip the baby colonies using microscopy or just magnifying glass 

163

Hi Ezekiel

Some useful references for you in attachment 1

https://www.coloss.org/beebook/II/fungal/1/4/1/3

2

https://www.mycobiology.or.kr/Synapse/Data/PDFData/0184MB/mb-32-197.pdf

3

https://www.fungaldiversity.org/fdp/sfdp/FD_2_47-63.pdf

4

http://bugs.bio.usyd.edu.au/learning/resources/Mycology/Growth_Dev/axenicCulture.shtml

5

http://bugs.bio.usyd.edu.au/learning/resources/Mycology/Growth_Dev/hyphalElongation.shtml

6

http://www.biotechniques.com/BiotechniquesJournal/2010/January/Isolation-of-fungal-homokaryotic-lines-from-heterokaryotic-transformants-by-sonic-disruption-of-mycelia/biotechniques-184894.html

7

http://www.wsrjournals.org/download.php?id=409908588654597278.pdf&type=application/pdf&op=1

8

http://website.nbm-mnb.ca/mycologywebpages/Moulds/Contamination.html

9

https://www.usask.ca/biology/kaminskyj/342/lab/Biol342_04lab/Ex2_Microfungi.pdf

10

https://books.google.iq/books?id=4yb-VnjZTycC&pg=PA65&lpg=PA65&dq=Isolation+of+Fungi+using+the+hyphal+tip+method&source=bl&ots=TpVj9tBz7s&sig=NbSo_lNoXNUdVyzpO443DNHO33g&hl=en&sa=X&redir_esc=y#v=onepage&q=Isolation%20of%20Fungi%20using%20the%20hyphal%20tip%20method&f=false

11

https://books.google.com/books?isbn=8120306686

12

https://books.google.com/books?isbn=0824705912

13

https://books.google.com/books?isbn=1461423562

14

https://books.google.com/books?isbn=3662061015

15

https://www.plantmanagementnetwork.org/pub/php/brief/2009/spore/

16

https://www.tandfonline.com/doi/full/10.1080/21501203.2012.727484

17

https://books.google.com/books?isbn=0080470262

18

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816652/

19

https://books.google.com/books?isbn=0520014979

20

https://books.google.com/books?isbn=1439804206

Culture your expected fungi on PDA or on your expected media. Incubate at 30-34 C for 24 hour at dark condition for first growing fungi or more than two days for slow growing fungi. Observe under microscope and cut your fungal hyphal tip after dichotomous branching or simply you can cut most extended part. Transfer to new plates. this technique you can use also to get rid of bacterial contamination as bacteria is comparatively slow grower.