How to isolate the cellulose Decomposing bacteria from Sugarcane field soil? If I combine soil with sugarcane straw, after how long time cellulose bacteria will be work and I can isolate? Soil and sugarcane straw mixture put into plastic bag or bowl? any have idea please suggest me.
Muhammad this paper could help! Let me know if you need more information
http://www.nature.com/articles/srep25279
Find attached link of our publication on screening of microorganisms for cellulases.
https://link.springer.com/article/10.1007%2Fs00284-008-9276-8
hello, Muhammad Tayyab.
when we want to isolate some bacteria with desired activities, we try to search them in some specific area , so now you want bacteria able to degrade cellulose from sugarcane field soil, now you can take a piece of decay sugarcane from soil or soil samples from different location, and put it in physiological water, after you can do serial dilution in LB agar medium, and you will isolate some bacteria so that you can test their cellulase activities in an appropriate colored agar medium also Characterization by sequencing the amplified region of 16S DNAr .
you can find a pic of this activity attached
http://pubs.sciepub.com/ajmr/2/1/6/
Cellulolytic bacterial strains were isolated from soil by using serial dilutions and pour plate technique. The me- dium used for isolation of cellulolytic bacteria contains 1.0 % peptone, 1.0 % carboxymethylcellulose (CMC), 0.2 % K2HPO4, 1 % agar, 0.03 % MgSO4.7H2O, 0.25 % (NH4)2SO4 and 0.2 % gelatin at pH 7 for 48 hours of in- cubation at 30 ̊C , Bacterial colonies were purified by repeated streaking. The purified colonies were preserved at 4oC for further identification and screening for cellulase production.
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