What is the best method for isolating HDMEC from human skin biopsies? Size of biopsy 8-12mm.
I hope that following pablication will be helpful.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1820617/
I hope to bring more robust HAES programing to the local community college in Washtenaw County and community. Is anyone aware of resources for health behavior and education programing on HAES? I...
08 June 2023 3,580 2 View
It is a rice genotypes evaluation and I want to use 1000 grain weight, but these genotypes can not produce 1000 grain yield due to drought so I want to scale down to a 100-grain weight. What is...
11 February 2023 3,458 3 View
Hi, I'm using all top ten models from the CABS_dock output directory (model1-10.pdb) but need to find the scoring metric for each model that ranks them accordingly - does anybody know what these...
01 February 2023 9,514 0 View
(Attached is the command inputted and error being thrown.) I'm getting an error while running adcp that occurs soon after warnings stating that adcp cannot locate any of the map files. However,...
11 January 2023 5,921 1 View
Just how wondering how widespread these schemes are.
21 July 2022 5,735 0 View
Hi, I'm interested in solving a nonconvex optimization problem that contains continuous variables and categorical variables (e.g. materials) available from a catalog. What are the classical...
31 March 2021 2,959 19 View
Hi, I'm running BARON on an AMPL instance that I uploaded on the NEOS server. Unfortunately, it times out after a few minutes. It is possible to pass an option file to set the time limit...
28 March 2021 5,071 1 View
Hi, I'm a researcher in optimization and a hobbyist photographer, and I'd like to get acquainted with lens design via the use of optimization methods. I found for example the paper...
22 September 2020 3,183 3 View
what stats to use for: 1. 3 metric dependent variables measured on one group of people in two conditions to see if the conditions effect the 3 metric variables (repeated-measures ANOVA?) 2. 1...
12 April 2020 4,376 3 View
Hello all, I've recently been attempting to synthesize monodisperse diameter gold nanoparticles (approx 2.5nm) based on the work of Levi-Kalisman (2019). In short, particles are synthesized...
24 February 2020 9,282 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View