Isolated some pathogenic non capsular NMeningitidis satrains?

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Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

What is your preference regarding Artificial Intelligence apps/methods/platforms for image analysis?

Please, let me know the apps, platforms, or methodologies based on AI to analyze images, such as radiographic or histology images. Tell me your experience of using AI in assessing patterns,...

30 June 2024 4,430 4 View

About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

14 June 2024 3,254 2 View

¿Cuánto conoce usted sobre sistemas de humidificación para pacientes en ventilación mecánica?

A través de este link que figura a continuación lo invitamos a participar del estudio "Encuesta de Enfoque Doble con Énfasis en Sistemas de Humidificación de Calentado...

09 June 2024 8,350 0 View

Are there any references available about experimental data on the sticking coefficients of atomic neutral H on Cu surfaces?

I have been searching for experimental data on the sticking coefficients of atomic neutral hydrogen on copper surfaces as a function of the initial kinetic energy of the beam, but I have only...

06 May 2024 8,714 1 View

What are the best research documents on process management in construction?

I am looking for research documents on process management in the construction sector. Articles that address topics related to continuous improvement, Kaizen, among others.

09 April 2024 9,467 1 View

Is there a lot of difference between standard LFP batteries and the LFP used in ev cars?

Apart from the size I can´t find any information about the LFP batteries used in BYD ev, for example. Can the size affect the discharge of tha battery or other characteristics?

19 March 2024 2,229 1 View

¿Cómo se aborda metodológicamente desde el trabajo social el estigma y la discriminación asociados a las drogodependencias?

Con esta pregunta se pretende identificar los métodos y técnicas más adecuadas para abordar las razones por las que las personas drogopendientes totalmente rehabilitadas, se encuentran en...

10 March 2024 6,561 2 View

Does anyone knows of a free software for reading STR in a 3500 analyzer (Applied Biosystems)?

We are mounting the STRs technique on an applied 3500 device (Applied Biosystems), which we usually use only for human identification (HiD). It turns out that we realize that reading STRs requires...

03 March 2024 949 1 View

Alternating Co-polymer through DFT?

Hi, I am a begineer in DFT calculations and I need to characterize an alternating co-polymer. I would like to know if you have some suggestions or additions on how to achieve this. I have just...

04 February 2024 9,668 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Have you heard about Technological Surveillance on Oceanographic Research Vessels?

It is important to highlight that technological surveillance on oceanographic vessels is a collaborative effort in which different specialists work together to effectively leverage technology in...

31 July 2024 1,633 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View