24 July 2013 1 6K Report

I have always used VectorNTI, I am happy with it, I am asking because I have not used anything else and am curious if anyone has ever compared it other programs.

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How can document analysis be best used in qualitative research?

I am using document analysis to explore how Christian philosophies of education are presented.

14 February 2024 9,867 2 View

Can you provide me with an efficient plasmid extraction protocol from yeast?

I've been trying to isolate a low copy (CEN/ARS) plasmid from yeast that has selective markers for both yeast and bacteria (Trp and Amp). However, when I try to recover the plasmid I can't get any...

20 October 2023 6,749 2 View

I'm working on an Algorithmic System for Identifying Specific Higher Gravitational Potential Values in a 3D Density Inversion?

The density currently being trained on all the reference models I've been looking at have a density range of 0.6-1.6 g/cm3 however were looking to identify values in the range of 19.3 g/cm3. Were...

27 July 2023 9,244 0 View

What impact would a distant sidechain mutation make to a drug docking calculation?

I would appreciate people's thoughts on a methodology consideration. We're docking several thousand small molecules at a specific location on the receptor. We're using Autodoc Vina to reduce the...

30 April 2023 5,659 3 View

Why should an "Off the shelf" wget/curl ZINC20 not work?

I've downloaded a text file full of curl and wget (two separate files) commands to download PDBQT files from ZINC20. However, none of the commands work. I've tried it on two machines using...

13 April 2023 269 1 View

Thromboelastography (TEG) or Calibrated Automated Thrombinography?

When assessing the contribution platelet concentrates make to coagulation, the above tests are commonly used within the platelet concentrate research field. However, are both nescisary for a...

21 November 2022 4,737 2 View

How to quantify seasonal drought?

I have time series data that I collected on soil moisture using sensors deployed at 27 sites, with data logged every 15 minutes. I am wondering if there are well defined metrics to quantify the...

18 September 2021 625 12 View

RNA extracts brown from plant roots?

I am extracting RNA from poplar roots using the sigma Spectrum Plant Total RNA kit and finding that the extracts are colored brown at the end of the protocol. Using an additional Zymo RNA cleanup...

01 September 2021 7,736 3 View

Percent moisture on very small tissue samples?

I am doing RNA extractions on plant roots which I plan to grind in liquid N prior to extraction. For my purposes, I want to precisely know the dry mass of the tissue that I extract, which means...

29 August 2021 8,231 3 View

When carrying out non-hierarchical cluster analysis on presence/absence data in PRIMER is it necessary to do both Bray Curtis & Jaccard similarities?

I am carrying out non-hierarchical cluster (kRCluster) analysis on presence/absence data in PRIMERv7. Is it necessary to do both Bray Curtis & Jaccard similarities? I have seen both carried...

05 July 2021 9,205 4 View

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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

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PCR showing no bands - Master mix and primers didn't mix?

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Dimensions of an MJ Research 96-well alpha module?

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How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

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Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

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Illustra™ MicroSpin™ G-25 columns what it is used for?

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How do I run a dead well test for a QuantStudio 5 qPCR machine?

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