Is using a rabbit primary antibody in rabbit reticulocyte lysate a problem?

Tobias Benjamin Schuster @Tobias_Schuster

03 March 2013 0 10K Report

Are there possible cross reactions or background using rabbit primary antibodies to detect in vitro translated human proteins from mRNA in rabbit reticulocyte lysate?

Badges
Science topic

More Tobias Benjamin Schuster's questions See All

What is the best way to carry out preparation of a cholesterol solution?

What is the best way to dissolve cholesterol to check the interaction with recombinant proteins in vitro and with proteins in cell lysate?

04 May 2014 9,940 5 View

Any suggestions for electroporation parameters using Biorad Gene Pulser Xcell to transfect mouse melanoma cells B16-F0?

Electroporation of mouse melanoma cell line B16

06 July 2013 5,594 0 View

How do you analyze secretion of a protein expressed in in vitro tranlation with rabbit reticulocyte lysate?

What proteins can I use as positive control for secretion?

02 March 2013 7,067 0 View

Does anyone know a good tutorial for using R in mass spec data sets?

I want to manage and analyse my data sets from mass spec experiments with the program R. I'm just a beginner in R, so I would be happy for any suggestions.

09 October 2012 1,779 2 View

Unable to detect biotinylated antibody with ExtrAvidin 1:200 by Western Blot?

I used this biotinylated antibody as a control for HRP-conjugated ExtrAvidin to detect biotin by westernblot. But now after incubation of the membrane with Luminol for 5 min I can visually see...

07 August 2012 977 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View