Tobias Benjamin Schuster

6 Questions 11 Answers 0 Followers

Questions related from Tobias Benjamin Schuster

What is the best way to carry out preparation of a cholesterol solution?

What is the best way to dissolve cholesterol to check the interaction with recombinant proteins in vitro and with proteins in cell lysate?

05 May 2014 9,980 5 View

Any suggestions for electroporation parameters using Biorad Gene Pulser Xcell to transfect mouse melanoma cells B16-F0?

Electroporation of mouse melanoma cell line B16

07 July 2013 5,627 0 View

Is using a rabbit primary antibody in rabbit reticulocyte lysate a problem?

Are there possible cross reactions or background using rabbit primary antibodies to detect in vitro translated human proteins from mRNA in rabbit reticulocyte lysate?

03 March 2013 9,608 0 View

How do you analyze secretion of a protein expressed in in vitro tranlation with rabbit reticulocyte lysate?

What proteins can I use as positive control for secretion?

03 March 2013 7,099 0 View

Does anyone know a good tutorial for using R in mass spec data sets?

I want to manage and analyse my data sets from mass spec experiments with the program R. I'm just a beginner in R, so I would be happy for any suggestions.

10 October 2012 1,810 2 View

Unable to detect biotinylated antibody with ExtrAvidin 1:200 by Western Blot?

I used this biotinylated antibody as a control for HRP-conjugated ExtrAvidin to detect biotin by westernblot. But now after incubation of the membrane with Luminol for 5 min I can visually see...

08 August 2012 1,010 2 View