Due to the Covid -19 , I'm unable to get supply of chemicals. At the moment, I have only left Trypan Blue with me. Is it possible for me to use Trypan Blue to stain fungus? Please help me with my doubts?
Hi Banupriya,
of course you can, and in many cases, this will be the first choice. Check Boedjin's original publication on this regard.
Article Trypan Blue as a Stain for Fungi
best
Francisco
Hi friend,
The answer is yes.
Several authors have used 'trypan blue' for staining fungi and the earliest paper I found dates back to 1952.
https://www.tandfonline.com/doi/abs/10.3109/10520295609113788?journalCode=ibih19
Some other papers:
http://m.cshprotocols.cshlp.org/content/2008/8/pdb.prot4982.abstract
https://pubmed.ncbi.nlm.nih.gov/21356882/
Hope this helps !
Thank you
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