Hi Banupriya,

of course you can, and in many cases, this will be the first choice. Check Boedjin's original publication on this regard.

Article Trypan Blue as a Stain for Fungi

best

Francisco

Hi friend,

The answer is yes.

Several authors have used 'trypan blue' for staining fungi and the earliest paper I found dates back to 1952.

https://www.tandfonline.com/doi/abs/10.3109/10520295609113788?journalCode=ibih19

Some other papers:

http://m.cshprotocols.cshlp.org/content/2008/8/pdb.prot4982.abstract

https://pubmed.ncbi.nlm.nih.gov/21356882/

Hope this helps !

Thank you