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Chromatography method development is mostly a logical mathematical process with very little subjective aspects.
One problem w the BOD bottle type of OUR measurement is that it can only be used in a system that is not DO limited (typically assumed about 2 mg/l or more). If the system is running at low DO and...
01 July 2024 1,282 2 View
Oxygen transfer in the activated sludge system involves physical mass transport across a two-film layer that consists of a gas film and a liquid film. For gases with limited solubility such as...
22 June 2024 5,044 0 View
I'm doing a Western blot and haven't seen any protein for two months. After transferring, the marker is visible, but when I detect the protein once, wash it, and try to detect another protein the...
10 June 2024 6,716 7 View
Currently, phenotypic characterization is routinely used in diagnostic laboratories for antibiotic resistance measurement due to its cost-effectiveness. With the advent of molecular diagnostic...
11 April 2024 4,022 4 View
activated sludge treatment plant treatment process
19 March 2024 10,005 4 View
We all know that in any gas-liquid dissolution interactions, under a constant rate of gas supply, the basic equation is given by: dC/dt = K1 (K2 - C) where K1 and K2 are arbitrary constants, dC/dt...
15 December 2023 6,429 9 View
I have deposited a layer of thiolate nanoparticles onto the substrate surface. Used dye and verified that SH group is active. Then I tried to use a maleimide/NHS ester crosslinker, but it seems...
16 November 2023 1,478 1 View
I have seen dosing in mg but never in ml. Any idea?
30 April 2023 4,108 0 View
Dear everyone, I am helping out a colleague to prepare an ERC starting grant and we are looking for successful applications from the social sciences and the humanities to hae an example....
13 April 2023 4,968 0 View
temperature correction factor is requested
03 February 2023 8,780 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View