Is transcardiac perfusion with PBS able to remove adherent leukocytes in blood vessels?

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How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

Is grammaticalization/subjectification a mechanism or motivation?

I know that we can categorize a specific linguistic change as either gramaticalization or subjectification (or both involved), but can we also say that specific linguistic change is motivated by...

31 May 2024 2,106 2 View

Could someone help a right procedure for TGA analysis of cement?

I analyzed cement samples exposed and unexposed to moisture over various periods. The TGA results of these samples showed variation with each repetition. However, I consistently used calcium...

22 May 2024 1,339 3 View

How many brain slices do we need to obtain 1 mg of protein for biochemical studies?

We are currently interested in performing a stimulation protocol on brain slices in order to perform western blotting or immunoprecipitation. However, we do not know how many 300 um slices would...

12 May 2024 6,527 1 View

Are you interested in W. Somerset Maugham?

We are organising a two-day international interdisciplinary conference on Maugham's life and works, in Le Mans, France on 13-14 March 2025. If you are interested, please read our CFP and send us...

12 May 2024 5,473 2 View

Does anyone have experience interpreting the output generated by Bottleneck software?

We are investigating the genetic structure of a population. We utilized the software 'Bottleneck.' Does anyone have experience interpreting the output generated by Bottleneck software?

17 April 2024 4,388 0 View

Can you identify what is this in my cell culture?

Hi, I have been cultivating Jurkat cells in RPMI supplemented with 10% FBS, and I have observed some small things in my flask. Although the cell growth appears unaffected, I want to ensure that...

16 April 2024 5,466 3 View

Solution Chloroplatinic acid?

What volume of Chloroplatinic acid is required to form 10 ml of a solution in which it is 16 mM? The density of Chloroplatinic acid is 2.43 g ml-1, and its molecular weight is 409.81. I really...

18 February 2024 7,908 2 View

Can i publish articles in reputed journals related to teachers as i am already working as an Assitant Profesor?

I am doing Ph.D studies on Organisational Citizenship Behaviour of University Teachers. I am also working as an Assistant Professor. I want to publish articles on OCB relating to Teachers. Can i...

24 January 2024 4,135 3 View

How to calculate DBH for trees with multiple dead and live stems in the same tree?

I am trying to calculate DBH (diameter at breast height) in order to determine the demography and basal area of trees. However, some of the trees have multiple stems which makes it difficult to...

23 January 2024 845 0 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View

"Hello, I am trying to find public datasets containing FTIR spectra of blood samples (both healthy and disease-related)?

These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"

17 July 2024 6,519 3 View

How to convert magnetic conductivity from SI to lattice units?

I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...

07 July 2024 5,243 0 View

Can we fix PBMCs in whole blood before we isolate them?

I am currently designing a study involving the examination of changes in RNA profiling of PBMCs before and after exercise. To achieve this, we need to collect whole blood samples before and after...

03 July 2024 2,891 1 View