Immunohistochemistry - How to do it?

Hi, I have got excellent results recently from my mice study on lung cancer xenograft tumors. I am looking to perform immunohistochemistry for analyzing the target proteins. We have never...

05 June 2019 9,095 4 View

Is 65% sequence identity good enough for basic modelling using Modeller?

I am trying to model a protein based on its homology. The two share 65% sequence identity. Is that good enough for basic modeling or should I opt for advanced modeling using Modeller? Btw, The...

02 March 2019 7,756 11 View

Can docking results from autodock using vina be published?

Hi, I have performed a docking study using my compound with a proteins obtained from pdb wesbite. I have used autodock vina for this. Can this results be published in a manuscript. It would be...

01 February 2019 1,276 10 View

Software for homology modelling?

I want to perform homology modelling for proteins from GLUT family. I have GLUT1 and GLUT3 (outward open conformation). Can someone suggest any software for generating homology modelled GLUT2...

01 February 2019 1,762 4 View

How to removes excess blocking?

I have been working with western blots and got great bands for actin. After that I stripped membrane with harsh stripping buffer but then blocked for 3hours (5% non-fat milk). I was not able to...

31 December 2018 3,296 7 View

Crispr ko cell line?

Hi, I generated a crispr ko cell line. The protein was more than 90% knocked out. But, now after several months od thawing and repassaging,the protein seems to comeback. I plan to do a grloth...

31 December 2018 9,505 10 View

How can I identify metabolites from LC-MS/MS data?

I just performed LC-MS/MS analysis. I have got a file with m/z, retention time, fragment score, etc. I want to know how can I do a pathway analysis and identify compounds? Which softwares...

11 December 2018 2,467 17 View

What is wrong with my transfer during western blot?

I tried to transfer today and found that the membrane (membrane 1) looked like this after ponceau S staining. Two days ago, I transferred two membranes together in the same cassette and found...

11 December 2018 2,545 7 View

Is my blot publishable?

Hi, I am trying to blot a protein called GLUT1. It is transmembane protein known for glycosylation. So I expected to observe streak rathen than a single band. This is what I got. I tried to KO...

08 September 2018 8,787 1 View

Tissue origin of HOP92 cells?

Hi, I am working on NCI60 cell line and my anticancer-compound supposedly targets cell lines form metastatic sites (Like MDA-MB-231, ACHN, SN-12C). I wanted to know if HOP92 also has its origin...

07 August 2018 1,909 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View