Is this mycoplasma contamination?

More Pratik Ramjivan Shriwas's questions See All

Immunohistochemistry - How to do it?

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Is 65% sequence identity good enough for basic modelling using Modeller?

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Can docking results from autodock using vina be published?

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Software for homology modelling?

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How to removes excess blocking?

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Crispr ko cell line?

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How can I identify metabolites from LC-MS/MS data?

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What is wrong with my transfer during western blot?

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Is my blot publishable?

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Tissue origin of HOP92 cells?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

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Why activated CAR-Jurkat cell could not kill targets?

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Could dyes amplify the spectrum of light to a specific wavelength?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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04 August 2024 7,282 2 View

Vilma Petrikaite

It seems that your cells might be contaminated. I would suggest you to use PCR reaction to detect mycoplasma in order to be sure.

Badreddine Mechri

Hi,

I'm totally agree with Dr. Vilma, it's clear that your cells are contaminated. I recommend this article, I hope that will be useful. Good Luck.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584481/

Cecilia Bucci

Unfortunately your cells look heavily contaminated. To investigate whether this is a mycoplasma contamination there are commercial mycoplasma detection kits based on PCR or antibodies.