How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

How to compile UDF for parallel processing ?

I am trying to use a UDF for velocity input in the room from a fan. I have created a UDF but when I am trying to load the UDF it shows an error stating that it is not compiled for parallel...

03 February 2021 2,187 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

Can you help me find a good, new (2009 and above) resources to get some informations and basics about plant systematic?

Hi, I hope you are doing well I'm a college student interested in botany and everything related to it. Lately I've been so into classification of plants and I don't know how to find a resource. I...

01 February 2021 1,549 5 View

How do you extract a specific chemical from a plant?

I know how to get the general extract from a plant - I can get the total phenols, alkaloids, Flavanoids, etc, but I want to isolate just one particular phenolic compound from the total extract....

21 October 2020 6,384 19 View

Can anyone give me advice on choosing Mphil in Plant systematics?

I am currently an undergraduate student (major in biological science) who is looking for a Master of Science in Plant Systematics and Evolution Biology aspects. My result in university is quite...

12 October 2020 1,559 3 View

How plant taxonomy is the mother of botany?

Research articles

11 October 2020 380 19 View

Who can share me otostegia integrifolia cultivation guideline or related material?

This is to know botany, ecology and cultivation practices.

09 October 2020 5,317 2 View

What is the relationship between plant hormones and plant allometry?

Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...

21 September 2020 9,653 6 View

What are the criteria one must look into while searching for a research guide?

As we know that guide plays a vital role in the entire tenure of research leading to PhD, what are the qualities a scholar must look into consideration while selecting a guide?

19 September 2020 2,538 18 View

Zootaxa, a one-time problem or an announcement of troubles in the future?

Dear all taxonomists Do you think that the problem which we had with Zootaxa in this year is an accidental or we should expect similar problems in the future? Not only for Zootaxa but also with...

13 September 2020 3,391 22 View

For how many days a human can survive in Subtropical Broad-leaved Hill Forests with 500 calories of veg and how?

Subtropical Broad-leaved Hill Forests such as Assam sub-tropical hill savannah woodland in India has a wide range of vegetation and is home to thousands of kinds of herbivores. What if a human has...

27 July 2020 2,350 7 View

Can anyone suggest a PhD dissertation area that encompass Microbiology and Immunology?

Can anyone please suggest some related topic or area in microbiology and immunology except COVID/SAARS?

04 July 2020 4,962 37 View