Can I use a causal model for representing subjective qualitative findings?

I have conducted grounded theory research via mixed methods approach. The study was conducted in two phases. First phase was purely grounded and so ended up while developing core categories and...

05 January 2021 505 6 View

What are the conditions in which researcher is unable to decide or calculate sample size for quantitative data?

Hello everyone! Is it statistically correct to conduct quantitative survey via convenience sampling, without deciding sample size from the population? I would like to add here that the study is...

16 September 2020 4,241 3 View

Autodock Vina on CUDA GPU?

How its possible to run Autodock Vina on dedicated GPU in windows. I want to make virtual screening faster by making use of GPU. Is there any application that can make is simple. I tried to make...

09 May 2020 3,330 5 View

What are the experimental methods to measure magnetic field, B of a novel electromagnet?

I am following the below method so far & need guidance to further modify it: 1) Making a specimen 2) Making an Electromagnet using prepared specimen 3) Using DC & AC Source to find BH-...

06 April 2020 3,779 5 View

What are the methods of calculating electromagnetic properties of Composites?

As intended composite is novel, so I want to know is it possible to calculate magnetic values by placing it's sample in some solenoid. Where I want to check magnetic field strength by changing...

01 March 2020 2,278 4 View

Can I construct a gus reporter vector with genepromotr-5utr-genomic seq(introns included)-3utr----gus ?

I have constructed and transformed my gus construct in the following way construct : gene promotor-5utr-genomic seq(introns included)-3utr----gus . My gene produce two kind of transcripts. So...

24 October 2019 4,382 2 View

Collection of Silymarin Gold Nanoparticles?

Hi, I am working on synthesis of Silymarin Gold Nanoparticles for my project and I am unable to get nanoparticles in dry powder form for characterization tests. Can anyone kindly guide me with...

10 August 2018 4,460 2 View

How to develop and validate a Mindfulness assessing instrument?

The literature so far ensues the probable discrepancies in the present mindfulness instruments either at conceptual level or psychometric level. I am going to be conducting my doctoral research in...

25 July 2018 1,697 5 View

Media influence on radicalisation impacting young muslims in UK

I want to know how media portrayal of radicalisation has an impact on young muslims in UK.

28 March 2017 1,158 2 View

How to grow polyaniline nanofibres on polyurethane sponge with and without CNT coating?

27 January 2016 5,566 2 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Which photsynthetic machine is good to use for measruing photysnthesis and CO2 assimilation for Arabidopsis?

Hi all, we are looking to buy a photosynthesis meter that can potentially measure photosynthetic rate, stomatal conductance, CO2 assimilation, PS1 and PSII efficiency (if possible), etc. it would...

02 February 2021 5,223 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

Equation to calculate Chlorophylls and carotenoids concentrations from a MeOH:HCl 1% (v/v) plant extract solution?

Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....

25 January 2021 3,686 2 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Triggering feeding of beetles / how to make them hungry?

Hey, I am working with Psylliodes chrysocephala feeding on oilseed rape. In feeding trials I often have the problem that a huge portion of the beetles do not feed at all. In clip cages I observed...

19 January 2021 9,851 6 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View