Sehrish Sarfraz

3 Questions 2 Answers 0 Followers

Questions related from Sehrish Sarfraz

How to amplify two unique alternative transcripts of single gene using one pair of primers?

In allotetraploid B.napus plant transcriptome, Based on pacBio 3rd generation sequencing, one gene has evidence of Alternative splicing (AS) in it. This gene has four exons and produces 2 distinct...

07 January 2022 8,878 4 View

Is there pacbio arabidopsis transcriptome data available? if yes can someone help me to find out?

I have to compare my Brassica transcriptome results with Arabidopsis.

30 July 2020 7,317 1 View

Can I construct a gus reporter vector with genepromotr-5utr-genomic seq(introns included)-3utr----gus ?

I have constructed and transformed my gus construct in the following way construct : gene promotor-5utr-genomic seq(introns included)-3utr----gus . My gene produce two kind of transcripts. So...

25 October 2019 4,456 2 View