What selection markers to use for the generation of stable HEK293 cell line ?

Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...

14 August 2020 7,542 2 View

What are your approaches and practices regarding games in the EFL classroom?

Dear fellow EFL teachers, we, Laura Schmetzer and Lennart Holzhuetter, are currently writing our master's thesis on the topic of games in the EFL classroom and would really appreciate it, if you...

28 July 2020 3,077 2 View

How can I export data (csv) from scopus effecient to an excel table?

Hey community i have a question for you. Im doing a literature review and i want to put the relevant literature in an excel table to check it later. Whenever i am exporting something in CSV (using...

13 January 2020 5,916 3 View

How does Burying beetle Nicrophorus vespilloides burying and copulating act look like?

I have read several papers about Nicrophorus vespilloides burying and copulation and I still have some questions. -Do only males bury the carrion? -Does male figth with intruders or also female?...

11 February 2018 8,481 9 View

Can you identify those bird feathers?

19 April 2017 2,715 3 View

Do I need permission to collect invertebrates from Gran Canaria?

I am going to Gran Canaria and planning to collect some invertebrates. Do I need permission for that and what should I do for getting the permission? I will collect for natural history museum of...

14 January 2017 5,777 15 View

Would you please identify those freshwater aquarium species?

20 October 2016 5,370 7 View

Can you identify those fish?

Please help me identify those fish. My oppinion 1-3 Taurulus bubalis 4-5 Cyclopterus lumpus

16 June 2016 725 6 View

What species of Mollusca are these specimens?

That species of Mollusca was found from lake Viljandi (https://www.google.ee/maps/@58.3483153,25.6106634,14z?hl=en). This summer. The measurements of shell are shell height 4,5mm shell width...

09 December 2014 2,540 12 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View