Ligands with 3,4,5-trihydroxyphenyl group?

Hi In the design of a bidentate ligand with imine group, is it okay to have a 3,4,5-trihydroxyphenyl group in the structure? In other words, does the 3,4,5-trihydroxyphenyl group compete with...

08 February 2021 4,977 7 View

Can anyone here guide how to write a good patent?

Dear Scientists I want to a person to guide me how to write a good patent.

08 February 2021 3,297 5 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

How can we assess professionalism for medical students in COVID-19 ear , when assess is being done online for most of the course?

Assessing professionalism is very challenging these days when most of assessment is online . We can declare students proficient un till unless we assess professionalism.

21 December 2020 2,467 3 View

What moment we can measure the polarization behavior by changing the external resistance in the Microbial fuel cell during the operation?

Hello Good Greeting for everyone I want help. What moment we can measure the polarization behavior by changing the external resistance in the Microbial fuel cell during the operation? If we are...

24 November 2020 7,100 2 View

Which parameter is the most important, removal % or adsorption capacity ?

I am testing a new material as adsorbent for the removal of methyl orange dye from aqueous solution, I got the below results, do I need to go further with higher concentrations even the removal...

05 November 2020 3,068 10 View

Helo everyone, I am looking for 4T1 cell for mouse model. from where can I get it at cheaper price?

I am trying to make syngeneic mouse model for TNBC for that I need 4T1 cell line. It is quite expansive commercially. So if anyone knows from where to get it or if someone can share it will truly...

15 October 2020 1,979 2 View

Amplification of a gene in Colorectal cancer clinical samples?

Dear all, Recently we tried to compare mRNA expression levels of a gene via real-time PCR in 40 colorectal cancer clinical isolates and 10 normal mucosa samples. Interestingly, a reasonable...

27 August 2020 9,022 1 View

I need to make animal model for TNBC.My institute lab do not have facilities for athymic/ nude or scid mice, can anyone guide to to proceed with this?

i want a xenograft MDA MB231 cell line animal model. can ER, PR and HER2 negative model be prepared by using an immunodeficient mouse model eg. BALB mice and which chemical can be used for this...

17 August 2020 7,279 3 View

question removed question removed?

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08 July 2020 7,344 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View