Dear All,
I am very interesting in controlling number of cell in droplet,
In this paper, they used statistic model to predict number of cell in the droplet
http://www.ncbi.nlm.nih.gov.ezlibproxy1.ntu.edu.sg/pmc/articles/PMC3524309/
Any other ways to control number of cell in a droplet ?
Appreciate all comments....
Best Regards,
Yannapol S.
It all depends if you want to know the precise number of cells or the average number of cells. Creating a homogenious cell suspension and then passing it through a syringe with different sized needles will allow you by counting the droplets and knowing the initial volume of the suspension to determine the average number of cells per droplet and manipulate the size of droplets by variations in the size of the needle. Changing in addition the variable of cell density of the suspension will allow you to manipulate the standard deviation.
Dear Yannapol:
Have a look at the work of Rogier Schoeman, e.g. High-throughput deterministic single-cell encapsulation and droplet pairing, fusion, and shrinkage in a single microfluidic device as appeared in Electrophoresis 2014.
It all depends if you want to know the precise number of cells or the average number of cells. Creating a homogenious cell suspension and then passing it through a syringe with different sized needles will allow you by counting the droplets and knowing the initial volume of the suspension to determine the average number of cells per droplet and manipulate the size of droplets by variations in the size of the needle. Changing in addition the variable of cell density of the suspension will allow you to manipulate the standard deviation.
Dr. Stoyanov is rightr. The exact same approach, seeding density, is used in encapsulation of cells with alginate hydrogel. The alginate droplet solidifies after leaving the needle tip and landing in a gellation bath. Once you collect the capsules, you can use a microscope to count how many cells are in each droplet. Then you can measure capsule diameter and using this to estimate Volume of Droplet (V_sphere = 4/3*pi*r^3) and to estimate your seeding density in droplet as a function of cell concentration of solution (cells/mL) vs cell seeding density_per capsule (cells/mL).
- Badges
- Science topic
- Similar topics
- Elementary Particle Physics
- Particle