One of my colleague is trying to isolate DNA in order to check a bacterial disease in olive. However she couldnt manage to isolate DNA up to now. She tried plant DNA isolation kit and CTAB buffer but after isolation she couldn't see any band in agarose gel. Also spectro results are not good. very limited amount of DNA and very bad purity. Do you have any experience DNA isolation from olive leaves or vein of the olive leaves?
Hi
Recommend using the kit on the link
http://eng.bioneer.com/literatures/manual/UG_MagListo%20Genomic%20DNA_EN_201604.pdf
Good evening,
I have never extracted DNA from olive leaves, but have extracted from plants with high amounts of secondary metabolites and phenolic. In the plants I couldn't get good results in I added 1mg PVP and 1mg activated charcoal before grinding leaves.
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