I got a problem in saving the NMR file?

The message I got from the computer was "vnmr2ucsf couldn't find the transmitter frequency for nucleus 1H in the procpar file". Please suggest the solution for this error.

08 January 2020 7,945 1 View

How we can do data collection and analysis?

How we can do data collection and analysis in the form of tabulation, statistical techniques and interpretation of those results.

07 January 2020 635 6 View

Can you suggest a Questionnair/Scale of Good Quality, available for free use on SELF MOTIVATION Analysis or alike theme?

I need to do a Pre and Post Reading Survey for a Motivational and Self-Help Novel. A Ready Made Questionnaire shall help. The same will be asked before they read and then after they have read. The...

05 October 2019 3,312 2 View

Can anyone help me with the interpretation of LCMS spectra?

Hi, I have seen many papers where the authors have deduced the molecular weight of protein/ compound directly from the LCMS spectra peaks. I also have an LCMS spectra but I am new to this...

16 September 2019 7,014 1 View

Does Graphene Quantum dots prepared by carbonization posses a Strong Raman SIGNAL?

For most carbon materials (Graphene, Graphene oxide, graphite oxide etc). we have combination of D-band (1350 cm-1), G-band (1580 cm-1) and G*-band (2700 cm-1) peaks of different ratios. For the...

02 July 2019 4,816 2 View

Reporting secondary data results of SmartPls 3?

Hi, Can anyone guide that which results of Smartpls 3 need to be reported in data analysis section when we use secondary data? As we get many results like reliability, validity, quality criteria...

01 November 2018 6,917 2 View

Research vs structural consultancy...which one should be considered for future and personal development??

Life is stuck in between consultancy and research work. Consultancy gives you practical experience along with money, however, research is limited to citations, projects etc. I know both fields are...

18 October 2018 7,373 3 View

Issue in SPSS for pattern matrix?

In pattern matrix in SPSS the loading's are good but same variables are not loading into single factor. what should i do to resolve it?

18 September 2018 3,279 6 View

How can i indexed my journal with google scholar?

we are running a journal. how can i indexed it with google scholar?

06 July 2018 1,186 6 View

Which medium is best for beas-2b cell line???

Hello can anyone tell me how to make complete medium with lhc-9 medium?? i want to start my work in beas 2b cell line..can anyone plz help me out/..how to make complete medium?? do we have to...

17 June 2018 3,693 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to reduce or increase CFU/ml value ?

I collected the water sample from pond and 1 ml of pond sample diluted with 9ml with water and 100 microliter was cultured into the MacConkey agar plates .Finally,80 colonies were formed in the...

24 January 2021 1,925 3 View