Is there anyone who can share with me the pressure range of a column of waters SYMMETRY C18, 3.5 µm 4.6 X 75 mm?

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

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Can you connect an HPLC to a Mass Spec only at a certain time point?

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GC-MS retention index prediticon?

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Can I use a HisTRAP column for affinity chromatography?

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Why is TLC not a form of partition chromatography?

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Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

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Is there a good freeware software for photodocumentation of TLC?

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How to improve peaks separation in HPLC?

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Gas chromatography RT detection?

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Problems with ion exchange chromatography?

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Dhaval Parekh

Hi..

As u mention column particle size in 3.5µm,natuarally pressure will be high.4000psi is still ok,but it should not exceed 5000psi.

Amir Haider

Mr.Dhaval Parekh have you experience of using same column.

Teodoro S Kaufman

Don't ever go above 6000 psi; common range is 3500-4000; sometimes less

Yes sir

Ok thanks