is there anyone know if the retroviral vector pMSGV1 can be packaged with psPAX2 and pMD2.G which is commonly used in lentivirus produciton？
Dear Linchun
Greetings,
As long as I'm concerned, if the pMSGV1 vector has the Psi sequence which is necessary for packaging, as well as 3' LTR and 5' LTR which are the integration site of gene of interest to the target cells genome, your vector cab ne packaged by psPAX2 and pMD2.G and would be suitable for cell infection.
Best,
Farhang
Hi, Farhang
Thank you for your kindly concern. the pMSGV1 has Psi sequence as well as 5',3'-LTR. but I saw a FAQ in Addgene:
"What is a lentivirus? How is it different than a retrovirus?
Lentiviruses are a subtype of retrovirus. The main difference between lentiviruses and standard retroviruses from an experimental standpoint is lentiviruses are capable of infecting non-dividing and actively dividing cell types, whereas standard retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and standard retroviruses use the gag, pol, and env genes for packaging. However, the isoforms of these proteins used by different retroviruses and lentiviruses are different and lentiviral vectors may not be efficiently packaged by retroviral packaging systems and vice versa."
I can not understand if the gag, pol,rev, tat gene on psPAX2 plasmid also work for retrovirus(pMSGV1) packaging since Addgene says they maybe different "isoform"
Best,
Linchun
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