Linchun Jin

5 Questions 9 Answers 0 Followers

Questions related from Linchun Jin

Retrovirus GAG gene primer and probe sequence for RVV titration by qPCR

Hi, Is there anyone know the qPCR primer and probe sequence for retrovirus GAG gene? I have qPCR primer and probe for lentivirus GAG. However, these two types of virus have different isotypes of...

14 March 2017 4,004 0 View

About control cohort in luciferase based killing assay?

I purchased Bright Glo (#E2620) for luciferase based killing assay. I co-cultured my effector T cell with target tumor cells (stably express luciferase) with different ratio in 200ul T cell media...

13 November 2015 7,159 2 View

Is there anyone know if the retroviral vector pMSGV1 can be packaged with psPAX2 and pMD2.G which is commonly used in lentivirus produciton?

is there anyone know if the retroviral vector pMSGV1 can be packaged with psPAX2 and pMD2.G which is commonly used in lentivirus produciton？

22 July 2015 5,720 3 View

Can anyone suggest how to design reverse primer of BbsI restriction site ?

Hi,I have a plasmid, and I want to replace the gene in it with my GOI,The restriction enzyme site is XbaI(upstream) and BbsI(downstream)I can design Forward primer of my GOI like this:F : 5’-...

12 March 2015 255 2 View

How can I do the titration of the lentivirus with qPCR?

I have produced lentivirus with my gene of interest. the protocol refers to some papers(Kutner, R., Zhang, X.-Y. & Reiser, J. Production, concentration and titration of pseudotyped HIV-1-based...

05 March 2015 1,404 9 View